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Objective To investigate mutation rate and types of KRAS in colorectal carcinoma , and to analyze the relationship between KRAS mutation and clinicopathological parameters in patients with colorectal carcinoma ( CRC) .Methods Scorpions Amplification Refractory Mutation System ( ARMS) fluorescence quantitative PCR was performed to detect the mutations in codons 12 and 13 of KRAS and to correlate between clinicopathological characteristics and the presence of various KRAS mutations of colorectal carcinoma .Results KRAS mutations were identified in 518 patients ( 42.92%) , including G12D ( 197 cases, 16.32%) , G12V ( 125 cases, 10.36%),G12C(40 cases, 3.31%),G12S(29 cases, 2.40%),G12A(21cases, 1.74%),G12R(7 cases, 0.58%),13D(117 cases, 9.69%).Female patients had a higher KRAS mutation rate than male (46.21%, 238/515 vs.40.46%, 280/692, P<0.05 ) .KRAS mutation was significantly higher in right colon cancer (46.45%,131/282)and in rectal cancer(44.50%,255/573)than that in left colon cancer(37.50%,132/352) (P<0.05,P<0.05).Conclusions There are many types of KRAS mutations in colorectal cancer, and many mutation types exist simultaneously .The detection rate of KRAS mutation is higher in female CRC patients than in the males.The detection rate of KRAS mutation is significantly higher in right colon cancer and in rectal cancer than that in left colon cancer.
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Purpose This study was to investigate the clinical significance of MDM2 protein expression and gene amplification in atypical lipomatous tumour/well-differentiated liposarcoma (ALT/WDL).Methods MDM2 protein expression and gene amplification were detected by immunohistochemistry of EliVision two-step method and fluorescence in situ hybridization (FISH) in 58 cases of ALT/WDL.Results Immunohistochemical staining showed that the positive rate of MDM2 was 79.3％,MDM2 gene amplification rate by FISH assay was 96.5％.There was statistically significant differences between immunohistochemistry and FISH (P ＜ 0.05).Conclusion MDM2 protein expression and MDM2 gene amplification provide the basis for diagnosis of ALT/WDL.However,a positive rate of MDM2 by immunohistochemistry is nearly 80％,so we recommand to carry out immunohistochemical MDM2 protein detection in most conditions without FISH which can help in the diagnosis of most cases of ALT/WDL.
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AIM:To identify the expression of fermitin family homolog 2 (FERMT2) in hepatocellular carci-noma ( HCC) tissues and the effect of FERMT 2 on the cell growth and related protein expression .METHODS:Real-time PCR and immunohistochemistry were used to detect FERMT 2 expression in the HCC tissues .The technique of CRISPR/Cas9 was applied to construct stable FERMT2 knockout MHCC97H cell line.WST-1 assay and flow cytometry were used to measure the cell viability , cell-cycle distribution and cell apoptosis .Western blot was used to determine the expression of related proteins in the MHCC97H cells.RESULTS:In HCC tissues, the expression level of FERMT2 was higher than that in adjacent liver tissues (P<0.05).High expression of FERMT2 was significantly correlated with postoperative recurrence of tumor.Knockout of FERMT2 gene evidently inhibited MHCC97H cell viability and accelerated cell apoptosis .Mean-while, the expression levels of proliferating cell nuclear antigen , cyclins, cyclin-dependent kinases 2 and anti-apoptotic fac-tors were significantly downregulated in MHCC97H cells with FERMT2 knockout (P<0.05).CONCLUSION:FERMT2 may function as a promoter of hepatocarcinogenesis and progression via regulating the cell viability , cell-cycle distribution and cell apoptosis , which is related with the expression of cell cycle regulators and anti-apoptotic factors .
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Objective To explore the effects of application of standard operation procedure (SOP) for perioperative nursing in children with developmental dislocation of the hip (DDH).Methods From March to September 2015,a total of 59 children with DDH were provided SOP for perioperative nursing.We retrospectively analyzed 50 cases of children with DDH treated with routine perioperative nursing admitted between June 2014 and December 2014.We compared the completeness of preoperative preparation,the incidence of postoperative complications and the parental satisfaction between two groups.Results Application of SOP for perioperative nursing increased the completeness of preoperative preparation,decreased the incidence of postoperative complications,and the parental satisfaction was greatly improved.Conclusion SOP for perioperative nursing for children with DDH can standardize nurses' working procedure,making nursing practice procedure much more specific,specified and clear,and can better guide clinical practice.
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Objective · To investigate the effect of modified electroconvulsive therapy (MECT) on oxidative stress parameters in patients with bipolar disorder. Methods · Forty-three patients with bipolar disorder (case group) were enrolled that received MECT intervention for 6 weeks, and 49 healthy volunteers (control group) were recruited. Chinese versions of the 17 items Hamilton Depression Rating Scale (HAMD-17), Young Mania Rating Scale (YMRS) and Clinical Global Impression-Severity Scale (CGI-S) were used to assess the efficacy and side effects at baseline and after 6 weeks of treatment. The plasma levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were measured at baseline and after 6 weeks of treatment to assess the level of oxidative stress. Results · The serum MDA and GSH-Px levels of the case group were higher while the serum SOD levels of the case group was lower than that of the control group, and there was no significant difference in the serum CAT levels between two groups at baseline. MDA levels were higher in manic states than in depressed states, and they were positively correlated with the CGI-S scores. After MECT treatment, the CGI-S scores of patients decreased significantly, and the plasma MDA levels decreased significantly in manic and depressive states, but there was no change in other oxidative stress parameters. Conclusion · There was oxidative stress damage in patients with bipolar disorder, and the severity of the disease varied with the degree of damage. MECT improved the symptoms of the disease and decreased the level of plasma MDA, while there was no effect on the anti-oxidation index.
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Objective To explore the effects of application of standard operation procedure (SOP) for perioperative nursing in children with developmental dislocation of the hip (DDH).Methods From March to September 2015,a total of 59 children with DDH were provided SOP for perioperative nursing.We retrospectively analyzed 50 cases of children with DDH treated with routine perioperative nursing admitted between June 2014 and December 2014.We compared the completeness of preoperative preparation,the incidence of postoperative complications and the parental satisfaction between two groups.Results Application of SOP for perioperative nursing increased the completeness of preoperative preparation,decreased the incidence of postoperative complications,and the parental satisfaction was greatly improved.Conclusion SOP for perioperative nursing for children with DDH can standardize nurses' working procedure,making nursing practice procedure much more specific,specified and clear,and can better guide clinical practice.
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Objective · To investigate the effect of modified electroconvulsive therapy (MECT) on oxidative stress parameters in patients with bipolar disorder. Methods · Forty-three patients with bipolar disorder (case group) were enrolled that received MECT intervention for 6 weeks, and 49 healthy volunteers (control group) were recruited. Chinese versions of the 17 items Hamilton Depression Rating Scale (HAMD-17), Young Mania Rating Scale (YMRS) and Clinical Global Impression-Severity Scale (CGI-S) were used to assess the efficacy and side effects at baseline and after 6 weeks of treatment. The plasma levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were measured at baseline and after 6 weeks of treatment to assess the level of oxidative stress. Results · The serum MDA and GSH-Px levels of the case group were higher while the serum SOD levels of the case group was lower than that of the control group, and there was no significant difference in the serum CAT levels between two groups at baseline. MDA levels were higher in manic states than in depressed states, and they were positively correlated with the CGI-S scores. After MECT treatment, the CGI-S scores of patients decreased significantly, and the plasma MDA levels decreased significantly in manic and depressive states, but there was no change in other oxidative stress parameters. Conclusion · There was oxidative stress damage in patients with bipolar disorder, and the severity of the disease varied with the degree of damage. MECT improved the symptoms of the disease and decreased the level of plasma MDA, while there was no effect on the anti-oxidation index.